Long, long ago
Worked as a lowly Research Tech in London UK
Where I also did a PhD researching early mouse and human development
Using (embryonic) stem cells and molecular biology approaches
Then a brief sojourn in Germany before having my own laboratory in 1988 in Basel Switzerland where I stayed eating fondue and chocolate for ~7 years, working on growth factors controlling the earliest stages of mammalian stem development.
By 1995, I was lured to the big city of Berlin (Germany) to a Max Planck Institute where I help develop and use robotic systems in molecular biology and the genetic modification of mouse embryonic stem cells.
1999 brought me to the USA (yes, they let me in!) and a Californian Biotech startup Deltagen, where I perfected large scale DNA screening systems and helped generate nearly 1000 mouse KO’s (gene knockouts) and wondered why it was so cold at night.
As the Bioteck boom popped, I fled north to The Jackson Laboratory (JAX) in 2002 with the objective of developing and improving mouse production, with a focus on cryopreservation, genetics – gaining the title of “Senior Director, Technology Evaluation & Development”.
By 2010, I began working at JAX with a new technology with great promise, i.e. “Targeted nucleases” to genetically modify mice precisely.
And then of course since late 2012, the sharpest tool of them all, CRISPR/Cas9.
Currently my research centers on approaches to introduce DNA/genes efficiently and precisely directly into mouse zygotes (eggs) to make genetically modified, and at times “humanized” mice.
I publish in scientific journals regularly and have a number of issued patents for my work.